By james art Club captain George Thomason picked up a hamstring injury in training on Friday and is rated as a major doubt for the St Andrews game.
There are also concerns over defender Eoin Toal and goalkeeper Nathan Baxter, who both missed Saturday’s 2-1 win at Burton Albion and are yet to be cleared to return.
Bolton had hoped Toal would be able to play against Burton after complaining of dizziness during his Northern Ireland internationals against Belarus and Bulgaria.
The club have yet to confirm whether the central midfielder has been diagnosed with a concussion, which could lead to mandatory rest.
Baxter picked up a muscle injury playing against Shrewsbury before the international break but was due to return to training before the weekend.
Whites assistant manager Stephen Craney took the post-match press conference on Saturday night but was unsure whether any of the three players would be able to play a part against the League One leaders.
“We’re hoping for a clean bill of health, but we won’t know about the players who played in that game until Monday morning. Regardless, we go to Birmingham and we have to put in a good performance,” he said.
“I think George (Thomasson) is his tendon, but in terms of the length of time I’m not too sure, we have to wait for the medical team.
“And then again it depends on Nathan and the medical team, sometimes the players are on the grass and then they’re not, so we’re just waiting until they tell us they’re fully fit. Until we’re told otherwise, we’ll go with the team we have.
Wanderers moved up to 11th with a 2-1 win at Burton, with substitute goals from Aaron Collins and Randall Williams. The club are six points clear of second-placed Wrexham, with a game in hand and now a point outside the play-offs.
Birmingham, who spent an unprecedented £30m on transfers in the summer, have dropped points in just two of their opening 10 games.
Charlton Athletic are the only side to have beaten them so far, but Craney gave an upbeat message from the Bolton dressing room after the final whistle.
“We believe that if we play to our potential we can win any football game and we will go there positive and so will Birmingham. We have to go there knowing we can win the game,” he said.
“Yes, it will be tough, St Andrews is a busy place at the moment and they have got the queues up, it will be a good atmosphere, 30,000 fans.
“But we have to go there and show some personality and character. We’ve won four of our last five and we want to keep that up. When you win games, it shoots you up the table and that’s what we want.
“We’ll be prepared for Tuesday and it’s a game we’re really looking forward to, so hopefully we can go there and get a good result.”
Regarding Burton’s performance, Craney admitted that the afternoon should have been more comfortable than it turned out to be.
“It was just disappointing to concede a goal so quickly after going 2-0 up,” he said. “It made him a little nervous towards the end. Congratulations to the boys, they survived.
“We don’t want balls going into the box at this late stage, so can we put more pressure on the ball to stop that happening. But when they came in, I think we defended them pretty well.
“There are many aspects in which we can improve in this game, but we are happy with the three points.”