The 46-year-old American actor, who began starring alongside Rogen in the 1999 comedy show Freaks And Geeks before working with him on Pineapple Express, This Is the End and The Disaster Artist, was charged with a sex offense in 2018 .
Franco is promoting his post-war drama Hey Joe at the Rome Film Festival after being absent from Hollywood films for several years.
Asked by Variety if he was in a relationship with Rogen, he said: “No. I haven’t spoken to Seth.
“I love Seth, we had 20 great years together but I guess it’s over. And not for lack of trying. I told him how much he meant to me.”
The comedy film Zeroville, which Franco directed and starred in alongside Rogen, was released in 2019.
He previously said the allegations from several women were “inaccurate” but he did not want to “shut down” people who “don’t have a voice”.
But he told The Jess Cagle Podcast that it was “wrong” for him to sleep with students at his acting school, adding that “it wasn’t a major plan on my part.”
Franco also told Variety that he used to be a “workaholic” and wishes he had “appreciated” the good times, including being up for awards at the height of his career.
He added: “I’m kind of grateful because it gave me a chance to just do whatever private work and really change what I needed to change.”
Franco agreed to pay $2.2m (£1.6m) in damages in 2021 after being accused of inciting female students to take part in sexually explicit scenes on camera.
He was nominated for an Academy Award for Best Actor for 127 Hours in 2010 and directed The Disaster Artist, which won an Academy Award for Adapted Screenplay.